The process of propagation of pineapple plants in the laboratory when there is no stable source of embryos, will include 3 main stages: (1) De-sampling; (2) Multiplication of shoots; and (3) Split the tree. When there are stable embryos, only 2 stages of shoot multiplication and plant separation are needed. After the plants are separated and grown to the appropriate stage, they will be planted in the nursery before being transferred to the field.
The facilities for the pineapple tissue culture laboratory are listed by function as follows. The lists of equipment and tools are listed at basic level, the quantity depends on the actual capacity to be cultured. When the specific needs are determined, the capacity will be calculated and from there calculate the specific area, the number of transplanting cabinets, the number of planters and the number of personnel.
Purpose:
Area: contains enough equipment and tools as below, there is space for dispensing and washing tools. The area is about 25-50 m2.
Purpose:
Area: enough to accommodate 1 steamer and drying cabinet and space for tools after steaming and preparing for steaming. The area is about 25 square meters.
Purpose:
Requirements: moisture and mold prevention. Area: depending on the number of incubators and the number of shelves needed to meet the culture capacity.
Purpose: used to inoculate embryo clusters and separate plants into sterile culture bags in sterile incubators.
Requirements: moisture and mold prevention.
Area: depends on the number of incubators to be placed, ie depends on the capacity to be inoculated in 1 day. On average, each work can separate 2,000 trees/day.
Purpose: to raise plants to multiply shoots and raise seedlings until they are transferred to the nursery.
Requirements: the ambient temperature is stable at about 25 degrees Celsius, continuously throughout the growing process.